Itekhnoloji ye-Ultrafiltration yitekhnoloji entsha kunye nephezulu yokwahlulahlula.Ineempawu zenkqubo elula, inzuzo ephezulu yezoqoqosho, akukho tshintsho lwesigaba, i-coefficient enkulu yokuhlukana, ukugcinwa kwamandla, ukusebenza kakuhle, ukungabikho kokungcola kwesibini, ukusebenza okuqhubekayo kwiqondo lokushisa lokushisa kunye nokunye.Namhlanje, umphathi uYang ovela eBeijing wabuza malunga nesixhobo sethu sokucoca iprotein kwaye wanxibelelana ngokweenkcukacha ngetekhnoloji yethu.Ngoku, umhleli weqela le-Shandong Bona liya kwazisa ukusetyenziswa kwe-ultrafiltration ekuhlukaneni kweeprotheni kunye nokuhlanjululwa.
1. Iprotheni desalination, dealcoholization and concentration
Ezona zicelo zibalulekileyo ze-ultrafiltration ekuhlanjululweni kweeprotheni zikhupha ityuwa kunye nokugxila.Indlela ye-Ultrafiltration kwi-desalination kunye nokugxininiswa kubonakaliswa ngumthamo omkhulu we-batch, ixesha elifutshane lokusebenza kunye nokusebenza okuphezulu kokubuyisela iprotheni.Indlela yemveli yokukhutshwa kwechromatography yesteric yokususa izinto ezahlukeneyo kwiiprotheyini ithathelwe indawo yitekhnoloji yanamhlanje ye-ultrafiltration, eye yaba yeyona teknoloji iphambili yeprotein desalination, dealcoholization and concentration today.Kwiminyaka yakutshanje, itekhnoloji ye-ultrafiltration isetyenziswe ngokubanzi ekukhupheni ityuwa kunye nokubuyiselwa kweeprotheyini zexabiso lesondlo esiphezulu kwi-cheese whey kunye ne-soybean whey.I-Lactose kunye neetyuwa kunye namanye amacandelo kwiprotheni, kunye neemfuno zangempela zokugqiba ngempumelelo i-desalting, i-alcoholization kunye ne-concentration of proteins.Ukusetyenziswa kwetekhnoloji ye-ultrafiltration inokuphinda igxile kwi-serospecies immunoglobulins ukuhlangabezana nemfuno yokwenyani yesivuno seprotheyini.
2. Ulwahlulo lweprotheyini
Ukwahlulwa kweeprotheyini kubhekisa kwinkqubo yokwahlula icandelo ngalinye leprotheyini ngokwecandelo ngokomahluko weempawu zomzimba kunye neekhemikhali (ezifana nobunzima bemolekyuli, indawo ye-isoelectric, i-hydrophobicity, njl.njl.) yecandelo ngalinye leprotheyini kulwelo lokutya.I-Gel chromatography yenye yeendlela eziqhelekileyo ezisetyenziswayo zokwahlulwa kwe-biological macromolecules (ingakumbi iiprotheni).Xa kuthelekiswa ne-chromatography yendabuko, iteknoloji yokwahlula i-ultrafiltration inethemba elihle lokusetyenziswa kwi-fractionation kunye nemveliso yemveliso yeeprotheni kunye nee-enzyme ezinexabiso elibalulekileyo lezoqoqosho ngenxa yeendleko eziphantsi kunye nokukhulisa okulula.Iqanda elimhlophe yeyona nto inexabiso eliphantsi ukufumana i-lysozyme kunye ne-ovalbumin.Kutshanje, i-ultrafiltration ihlala isetyenziselwa ukwahlula i-ovalbumin kunye ne-lysozyme kumhlophe kweqanda.
3. Ukususwa kwe-Endotoxin
Ukususwa kwe-Endotoxin yenye yeendlela eziphambili zokusetyenziswa kweteknoloji ye-ultrafiltration kwi-protein yokuhlanjululwa.Inkqubo yokuvelisa i-endotoxin inzima kakhulu.Kwinkqubo yokusetyenziswa okusebenzayo, kuba iprotein yamayeza eveliswa yinkqubo yokubonisa iprokaryotic kulula ukuxuba kunye ne-endotoxin eveliswa kukuqhekeka kodonga lwe-bacterial cell, kunye ne-endotoxin, ekwabizwa ngokuba yi-pyrogen, luhlobo lwe-lipopolysaccharide.Emva kokungena emzimbeni womntu, kunokubangela umkhuhlane, ukuphazamiseka kwe-microcirculation, ukothuka kwe-endotoxic kunye nezinye iimpawu.Ukuze ukhusele impilo yabantu, kuyimfuneko ukusebenzisa itekhnoloji ye-ultrafiltration ngokubanzi ukususa i-endotoxins.
Nangona iteknoloji ye-ultrafiltration isetyenziswa ngokubanzi ekwahluleni nasekuhlambululeni iiprotheni, nayo inemida ethile.Ukuba ubunzima be-molecular yeemveliso ezimbini eziza kuhlukaniswa bungaphantsi kwamaxesha ama-5, abukwazi ukuhlukana nge-ultrafiltration.Ukuba ubunzima bemolekyuli yemveliso bungaphantsi kwe-3kD, ayinakugxininiswa yi-ultrafiltration, kuba i-ultrafiltration idla ngokuqhutywa kubunzima obuncinci be-molecular of the membrane kwi-1000 NWML.
Ngophuhliso oluqhubekayo lweshishini le-bioengineering, iimfuno eziphezulu zibekwe phambili kuluhlu olusezantsi kunye nobuchwepheshe bokucoca.Iindlela zemveli zokuxinana kwe-vacuum, i-solvent extraction, i-dialysis, i-centrifugation, imvula kunye nokususwa kwe-pyrogen ayifumaneki ukuhlangabezana neemfuno zokuvelisa kwakhona.Itekhnoloji ye-Ultrafiltration ibopheleleke ukuba isetyenziswe ngakumbi kwaye isetyenziswe ngokubanzi kuba iinzuzo zayo ekuhlukaneni kweeprotheyini.
Ixesha lokuposa: Apr-20-2022